Rabbit IgG Isotype Control (H+L)

This protein is purified from rabbit serum by immunoaffinity chromatography which removes essentially all rabbit serum proteins, except the specific rabbit IgG. Cited in 42 publication(s).

Product Info Summary

SKU: BA1045
Size: 10mg
Host: Rabbit

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Product NameAffiniPure Rabbit IgG (H+L)
SKU/Catalog NumberBA1045
Pack Size10 mg
Formlyophilized
PurityThe antibody was purified from antiserum by Protein A.
HostRabbit
ClonePolyclonal
Contents10 mg of affinity purified specific antibody (purification>99%). 0.01M PBS.
Usage MethodRecommended Dilutions: Optimal working dilutions must be determined by end users. Working solution can not be preserved for long after being diluted with 0.01M PBS buffer solution (pH7.0-7.6) or 1% sodium acetate. After adding preservatives (such as 0.05% NaN3) and reagent grade BSA to 1%, the antibody can be preserved for a long time. When labeling antibodies, such as HRP, FITC, Biotin, etc., the reagents should not contain BSA and NaN3.
StorageAt -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
ApplicationsIHC, IF, ICC, WB, ELISA
Cite This ProductRabbit IgG (H+L) (Boster Biological Technology, Pleasanton CA, USA, Catalog # BA1045)

Validation Images & Assay Conditions

Hello CJ!

BA1045 has been cited in 42 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Andrographolide plays an important role in bleomycin-induced pulmonary fibrosis treatment

Exendin-4 protects retinal cells from early diabetes in Goto-Kakizaki rats by increasing the Bcl-2/Bax and Bcl-xL/Bax ratios and reducing reactive gliosis

Expression of toll-like receptor 4 in uvea-resident tissue macrophages during endotoxin-induced uveitis

Survivin is a prognostic indicator in glioblastoma and may be a target of microRNA‑218

c-Jun N-terminal kinase inhibitor favors transforming growth factor‑β to antagonize hepatitis B virus X protein‑induced cell growth promotion in hepatocellular carcinoma

Herpes Simplex Virus Type 2 Immediate Early Protein ICP27 Inhibits IFN-β Production in Mucosal Epithelial Cells by Antagonizing IRF3 Activation

Herpes Simplex Virus Type 2 Infection-Induced Expression of CXCR3 Ligands Promotes CD4+ T Cell Migration and Is Regulated by the Viral Immediate-Early Protein ICP4

Ets-2 Regulates Cell Apoptosis via the Akt Pathway, through the Regulation of Urothelial Cancer Associated 1, a Long Non-Coding RNA, in Bladder Cancer Cells

Exendin-4 Induces Bone Marrow Stromal Cells Migration Through Bone Marrow-Derived Macrophages Polarization via PKA-STAT3 Signaling Pathway

Protective Effect of an Antibody against Specific Extracellular Domain of TLR2 on Agonists-Driven Inflammatory and Allergic Response

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1 Customer Q&As for Rabbit IgG Isotype Control (H+L)

Question

In which range have you tested it? A customer is getting unspecified signals at a dilution of 1:700 (--> 1,4

Verified Customer

Verified customer

Asked: 2019-11-08

Answer

The suggested dilution ratio of BA1045 is 1:1000. The non-specific staining might be caused by the secondary antibody (2nd antibody: goat anti-rabbit IgG ) or the binding between Strep HRP and endogenous biotin in the sample. We suggest the customer run a negative control by using PBS to substitute the primary antibody to see if there is any non-specific staining.

Boster Scientific Support

Answered: 2019-11-08

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